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KMID : 0613820120220030387
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Journal of Life Science
2012 Volume.22 No. 3 p.387 ~ p.397
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Synthesis and Biological Activities of Myomodulin A and Its Analogs
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Park Nam-Gyu
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Abstract
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In this study, we focused on myomoduline A (MMA) released from the central nervous system of Aplysia kurodai. The primary structure of MMA is Pro-Met-Ser-Met-Leu-Arg-Leu-NH₂. This peptide is the same as that of the myomodulin family peptide found in other mollusks. The purified MMA showed a modulating activity of phasic contraction on the anterior byssus retractor muscle (ABRM) of Mytilus edulis. In order to study the relationship between the structure and biological activity of MMA, we synthesized MMA, Des[Pro¹]-MMA, Des[Pro¹,Met²]-MMA, Des[Pro¹,Met²,Ser³]-MMA, and MME. The amino acid sequences of Des[Pro¹]-MMA, Des[Pro¹,Met²]-MMA, and Des[Pro¹,Met²,Ser³]-MMA were Met-Ser-Met-Leu-Arg-Leu-NH₂, Ser-Met-Leu-Arg-Leu-NH₂, and Met-Leu-Arg-Leu-NH₂, respectively. MMA and synthetic peptides were tested on ABRM in M. edulis as well as muscle preparations in Achatina fulica. At 1×10^-8 M or lower, MMA showed a potentiating effect on phasic contraction of the ABRM, but this peptide had an inhibitory effect at 1×10^-6 M or higher. Both MMA and its analogs stimulated a contractile response on the crop and a relaxed catch-relaxing response on the penial retractor muscle of A. fulica. These results suggest that Met-Leu-Arg-Leu-NH₂ in MMA is the minimum structure required for the regulation of the contraction of ABRM, as well as the reproductive and digestive activities of mollusks.
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KEYWORD
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Myomodulin A, analog, penial retractor muscle, anterior byssus retractor muscle
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